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Publicationsand product literature |
What Scientists Have to Say"PPS outperforms everything else we've used to generate peptides from complex protein mixtures." – Mike MacCoss PhD, University of Washington "PPS enhanced recovery of large hydrophobic proteins by reducing peptide interactions with plasma proteins and increasing solubility. PPS allows us to use a more sensitive protocol for analyte measurement." – Yan Wang, PhD, Amylin Pharmaceuticals  |
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PPS Silent Surfactant solubilizes membrane proteins and cleaves to eliminate detergent interference.Features
How It Works
PPS Silent Surfactant is acid-cleavable so it can be removed by hydrolysis. The intact molecule is zwitterionic, so PPS Silent Surfactant can also be removed by SCX HPLC. Performance
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Hydrolysis of PPS Silent Surfactant clears detergent interference. Acid hydrolysis of PPS Silent Surfactant clears detergent interference, enabling mass spectrometry analysis of peptides resulting from trypsin digestion of complex protein mixtures
Hydrolysis products are non-surfactant. Loss of surfactant property upon hydrolytic cleavage. Cleavage occurs within 30 minutes in 0.25 M HCl. "The proteins we study are tough to solubilize and digest, but PPS Silent Surfactant is strong enough to give us good digestions and recoveries. It is clear from our spectra that PPS Silent Surfactant does not interfere with subsequent labeling of peptides with iTRAQ." "The PPS reagent allows us to capture cells, efficiently extract proteins, and digest, all in a single tube that is directly compatible with downstream LC and MS analysis without the need for involved clean-up that would result in substantial losses of clinically valuable material." |
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