FASP™ Protein Digestion Kit

Complete Protein Solubilization. Complete Trypsin Digestion. Every Time.

Universal Sample Preparation with the FASP Protein Digestion Kit

Now you can use aggressive, whole proteome solubilization protocols and reagents to prepare proteins from any biological material for trypsin digestion and analysis by mass spectrometry. Based on the Universal Sample Preparation method developed by Wisniewski, Zoubman, Nagaraj and Mann, Protein Discovery’s FASP Protein Digestion Kit enables efficient digestion of samples for proteome analysis, even in the presence of the most extreme contaminating species. This method is the perfect complement to lysis buffers containing strong detergents and reducing agents. No longer are you forced to choose between efficient extraction or digestion. The FASP kit allows you to do both at the highest efficiency.

A Universal Method for Trypsin Digestion

Initially described by Manza, et al., and further developed by Wisniewski, et al., the concept of using a spin filter for cleanup and digestion of protein samples for mass spectrometry analyses offers several advantages over more conventional approaches. In FASP (Filter-Aided Sample Prep), the spin filter forms the basis of a “proteomic reactor” for sample preparation. This proteomic reactor is used to deplete SDS, salts, and other interfering substances from the protein sample prior to alkylation and trypsin digestion. Following digestion, peptides are eluted as a concentrated solution. High molecular weight interferences such as DNA remain trapped by the filter, so they stay out of the peptide solution filtrate.

Our Offer

The FASP Protein Digestion Kit is sold as a package priced at $49 US and contains columns, buffers, and other materials for eight digestions of up to .25 mg protein each. Your purchase also comes with our 30-day money-back guarantee. If you’re dissatisfied with the FASP Protein Digestion Kit for any reason, just send any unused materials back to us and we’ll refund your money promptly.

REFERENCES

Universal sample preparation method for proteome analysis. Wisniewski JR, Zougman A, Nagaraj N, Mann M. Nat Methods. 2009 May ; 6(5): 359-62

Sample preparation and digestion for proteomic analyses using spin filters. Manza LL, Stamer SL, Ham AJ, Codreanu SG, Liebler DC. Proteomics. 2005 May ; 5(7): 1742-5