UPX™ Universal Protein Extraction Kit

Protein Extraction with Complete Solubilization

Extract Membrane Proteins and Soluble Proteins for Mass Spectrometry Analysis

Protein Discovery’s UPX Universal Protein Extraction Kit is for researchers who need universal, unbiased extraction of both membrane proteins and soluble proteins, and an abundance  of starting material.

Extract Proteins at High Efficiency and Solubilize the Entire Proteome (click image to magnify).

One Protein Extraction Protocol for Tissues and Cells, Fresh or Frozen.

Protein Discovery’s UPX Protein Extraction Kit work with high efficiency on a wide variety of samples such as heart, liver, brain, and kidney organ tissues, mammalian-derived cultured cell lines, and bacteria. You can begin with freshly harvested or frozen cells.

Unbiased, High-Yield Extraction (click image to magnify).

Compatible with SDS-PAGE, Gelfree^® Fractionation, FASP™ Digestion

The lysis buffer in the UPX Protein Extraction Kit maximizes the extraction of proteins from cells and tissues under conditions that are compatible with downstream methods for preparing samples for mass spectrometry. Containing reducing agents and SDS, the lysis buffer provides unbiased, total extraction of membrane proteins and soluble proteins from cells and tissue. Solubulized proteins in the resulting lysates can be analyzed by SDS-PAGE and fractionated by the Gelfree 8100 Fractionation System. Protein Discovery recommends Filter-Aided Sample Preparation (FASP) for detergent depletion and complete trypsin digestion of extracts prior to analysis by mass spectrometry.

Our Offer

UPX Universal Protein Extraction Kits start at $50 for a single Kit containing materials for processing 5 g of tissue, or 1.5 g wet cell pellet. Generous discounts apply to purchases of five or more kits. Note that UPX Universal Protein Extraction Kits come with our 30-day money-back guarantee. If you’re dissatisfied with your UPX Universal Protein Extraction Kit purchase for any reason, just send any unused materials back to us and we’ll refund your money promptly.