Products for Extraction
Protein Extraction with UPX Universal Protein Extraction Kit
Protein Discovery’s UPX Universal Protein Extraction Kit is for researchers who need universal, unbiased extraction of both soluble and insoluble proteins, andlots of protein starting material. Read more about universal protein extraction with the UPX Universal Protein Extraction Kit.
Protein Extraction with YPX Yeast Protein Extraction Kit
The robust and diverse cell wall structures of yeast contribute to their ubiquity and durability, and the mechanical disruption protocols used by other methods to open yeast cells for extraction undoubtedly contribute to their characteristically low yields. Unlike those protocols, protein extraction with Protein Discovery’s YPX Yeast Protein Extraction Kit requires only two solutions, is easy to carry out on a large number of samples, and yields a surprising amount of protein. Find out more about the YPX Yeast Protein Extraction Kit.
Protease Inhibition with Proteoloc Protease Inhibition Cocktails
The addition of protease inhibitors to lysis buffers used for protein extractions is an effective means of protecting extracted proteins from enzymatic degradation by endogenous proteases. Find out more about Protein Discovery’s Proteoloc Protease Inhibition Cocktails.
Protein Extraction from Cells and Tissue
Protein extraction from cells or tissue is often the first step in a laboratory workflow leading to proteome analysis by mass spectrometry. These workflows can be quite complex, and successful completion can require a substantial investment of laboratory time and materials. It is therefore extremely important to begin with an extraction method that will deliver proteins in sufficient concentration, diversity, and quantity to enable the workflow to finish successfully.
Protein Discovery’s extraction kits are designed in accord with the following criteria:
- High extraction efficiency to deliver a quantity of protein sufficient to produce strong, clear mass spectrometry signals after accounting for potential total protein losses over consecutive proteomics workflow steps
- Unbiased extraction with respect to protein size
- Unbiased extraction with respect to protein hydrophobicity
- Standardized extraction efficiency for reproducible quantitative proteomics
- Compatibility with downstream workflow steps leading to mass spectrometry
- Compatibility with a wide variety of sample types for benchtop convenience and laboratory economy
- Speed and convenience for high laboratory efficiency
- Rapid, simplified protocols to enable processing of many samples simultaneously
