FASP Protein Digestion Kit FAQ
Basics
What Does the FASP Protein Digestion Kit Do?
The FASP Protein Digestion Kit:
- Depletes contaminants from any protein sample solution
- Alkylates thiols
- Digests proteins in a spin-filter “proteomic reactor”
- Releases peptide products of digestion in a small volume filtrate solution
- Excludes remaining large molecules such as DNA from the digestion product preparation
When Should I Use the FASP Protein Digestion Kit?
Use the FASP Protein Digestion Kit whenever you’re concerned that SDS, reducing agents, salts, or other contaminants might interfere with trypsin digest efficiency.
How Can the FASP Protein Digestion Kit be Effective on a “Universal” Range of Samples?
The FASP Protein Digestion Kit depletes comtaminants such as salts, detergents, and reducing agents from the sample prior to alkylation and digestion.
Is the FASP Protein Digestion Kit Similar to the “Universal Sample Preparation” Method Described byWisniewski,Zougman,Nagaraj andMann?
The FASP Protein Digestion Kit enables researchers to successfully reproduce the Universal Sample Preparation method described by Wisniewsky, et al.
Why Does the FASP Protein Digestion Kit Come with Two Protocols?
The first protocol is the one to use on whole-proteome extracts and other cell or tissue lysates. The second protocol is for fractionated protein samples prepared using the Gelfree 8100 Fractionation System.
Purchasing Considerations
What Additional Materials Do I Need?
In addition to the FASP Protein Digestion Kit, you’ll need 30 μg of trypsin for each digestion and some common laboratory supplies and equipment. The package insert has a complete list for you to check before you start.
How Many Digests Can I Do with One Kit?
The FASP Protein Digestion Kitcontains sufficient materials for eight digests of up to 0.4 mg protein each.
What is the Shelf Life of the Kit?
The product shelf live of materials provided with the FASP Protein Digestion Kitis two years.