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The Gelfree 8100 Fractionation System is Essential Equipment for Top-Down Proteomics. Here's Why:
- Intact protein fractionation: The Gelfree System fractionates intact protein by molecular weight
- Liquid-phase recovery: No band or spot cutting means higher recoveries in less time
- Broad mass range fractionation: Select up to 30 fractions between 3.5 kDa and 150 kDa
- Small solution volumes: Even fractions containing large proteins are collected in a maximum volume of 150 µL
- Reduced sample complexity: Fractionation by molecular weight improves ionization of your proteins of interest and makes them easier to see
- 2-D clarity: Combine Gelfree size fractions with RPLC or solution-phase Offgel IEF.
- Used productively by top proteomics researchers: Neil Kelleher, Alan Doucette, Jeremiah Tipton, and more scientists use Gelfree systems for top-down proteomics research.
Recent Gelfree Publications
- Gel-eluted liquid fraction entrapment electrophoresis: an electrophoretic method for broad molecular weight range proteome separation. Tran JC, Doucette AA. Anal Chem. 2008 Mar 1; 80(5): 1568-73
- Multiplexed Size Separation of Intact Proteins in Solution Phase for Mass Spectrometry. Tran JC, Doucette AA. Anal Chem. 2009 Jul 2;
- A Robust Two-Dimensional Separation for Top-Down Tandem Mass Spectrometry of the Low-Mass Proteome. Lee JE, Kellie JF, Tran JC, Tipton JD, Catherman AD, Thomas HM, Ahlf DR, Durbin KR, Vellaichamy A, Ntai I, Marshall AG, Kelleher NL. J Am Soc Mass Spectrom. 2009 Aug 12;
Recent Gelfree Posters
- Top Down Proteomics Using Online Polymer Reversed Phase (PLRP) Nanocapillary-LC Coupled Fourier Transform Mass Spectrometry. Adaikkalam Vellaichamy; John F. Kellie; John C. Tran; Ji Eun Lee; Adam Catherman; Jaeyun Sung; Nathan D. Price; Gary Valaskovic; Neil L. Kelleher. Presented at 2009 ASMS conference.
- Molecular Weight‐Based Fractionation of Intact Proteins with Liquid Phase Recovery using the GELFREE 8100. Nghia Chiem, Christopher Dill, Jay Harkins, Peter Osucha, Chuck Witkowski, Jeremy Norris. Presented at 2009 ASMS conference.
- 2D Fractionation of Intact Proteins Prior to RPLC Coupled to LTQ-FT MS/MS. John C. Tran, Cong Wu, John F. Kellie, Ji Eun Lee, Adaikkalam Vellaichamy, Kenneth R. Durbin, Adam D. Catherman, Leonid Zamdborg, Paul M. Thomas, Neil L. Kelleher. Presented at 2009 ASMS conference.
Components
The Gelfree 8100 Fractionation System consists of the Gelfree 8100 Fractionation Station and Gelfree 8100 Cartridges.
| Gelfree 8100 Fractionation Station |
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Gelfree 8100 Cartridge |
How It Works
Gelfree 8100 Cartridges complement the Gelfree 8100 Fractionation Station, which delivers specified current or voltage to each of the eight channels and pauses for fraction collection according to user programming.
Gelfree technology is gel eluted liquid fraction entrapment electrophoresis.
Each of the eight Gelfree 8100 Cartridge channels consists of a gel column surrounded
by pipette-accessible sample loading and fraction collection chambers. Proteins eluted
from the column are entrapped in a defined volume of solution in the collection chamber.
A 3.5 kDa molecular weight cut-off trapping membrane repels negatively charged
proteins to limit protein-membrane binding interactions and optimize yield.
How much does it cost? Get pricing.
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